热带地理 ›› 2016, Vol. 36 ›› Issue (1): 108-113.doi: 10.13284/j.cnki.rddl.002801

• 论文 • 上一篇    下一篇

南海北部陆坡表层沉积物氨氧化古菌多样性初探

刘国辉1,3,吴后波2   

  1. (1.中国科学院边缘海地质重点实验室,中国科学院南海海洋研究所,广州 510301;2.中国科学院南海海洋研究所 海洋生物资源可持续利用重点实验室,广州 510301;3.中国科学院大学,北京 100049)
  • 出版日期:2016-01-05 发布日期:2016-01-05
  • 通讯作者: 吴后波(1967―),男,湖北洪湖人,副研究员,博士,主要从事海洋环境微生物研究,(E-mail)wuhoubo@scsio.ac.cn。
  • 作者简介:刘国辉(1982―),男,山东烟台人,博士研究生,主要从事海洋微生物分子生态学研究,(E-mail)sdlgbio@126.com
  • 基金资助:
    国家重大科学研究计划(2013CB956101);国家自然科学基金项目(91428203);中科院先导项目(XDA05080301)

Diversity of Ammonia-Oxidizing Archaea in the Surface Sediments of the Northern Continental Slope of the South China Sea

LIU Guohui1,3,WU Houbo2   

  1. (1.Key Laboratory of Marginal Sea Geology,South China Sea Institute of Oceanology,the Chinese Academy of Sciences,Guangzhou 510301,China; 2.Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,the Chinese Academy of Sciences, Guangzhou 510301,China;3.University of Chinese Academy of Sciences,Beijing 100049,China)
  • Online:2016-01-05 Published:2016-01-05

摘要: 基于南海北部陆坡不同深度梯度3个站位表层沉积物古菌氨单加氧酶基因(amoA)文库,对3个站位氨氧化古菌进行多样性和系统发育学分析。多种方法构建的系统发育树表明:3个站位所有的amoA基因序列都隶属于奇古菌门中Group I分枝内的Group I.1a系群,且各站位之间氨氧化古菌多样性没有明显的差异。501站位黑色砂质沉积物中古菌amoA基因与该站位的16S rRNA基因的系统发育比对显示:这2种基因标记的系统发育树整体上具有潜在的对应关系,说明对样品的氨氧化古菌多样性分析比较全面且可靠;并进一步暗示该样品中氨的硝化作用主要由奇古菌门下的Group I.1a系群来执行。由此可以推测:Group I.1a系群可能在南海北部表层沉积物中氮素的生物地球化学循环过程中扮演重要的角色。

关键词: 氨氧化古菌, amoA基因, 表层沉积物, 南海北部

Abstract: Surface sediment samples were taken from three sites of different water depths at the northern continental slope of the South China Sea to investigate the community structure of ammonia-oxidizing archaea (AOA). Polymerase chain reaction (PCR) was employed to amplify the archaeal ammonia monooxygenase α subunit (amoA) gene of AOA. Amplicons of the amoA gene sequences were used to understand the diversity and phylogenetic relationship of AOA. The main results were as follows: 1) All of the archaeal amoA gene sequences from the three locations were affiliated to Group I.1a Thaumarchaeota; 2) all of the archaeal amoA gene sequences from the three locations were phylogenetically closely related; and 3) the 16S rRNA gene and amoA gene phylogenetic trees were of congruent topology. Thaumarchaeota accounted for 40% of the total archaea, indicating that AOA might play an important role in the nitrogen biogeochemical cycling in the surface sediments of the northern continental slope of the South China Sea.

Key words: Ammonia-Oxidizing Archaea, amoA gene, surface sediment, northern South China Sea