TROPICAL GEOGRAPHY ›› 2016, Vol. 36 ›› Issue (1): 108-113.doi: 10.13284/j.cnki.rddl.002801

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Diversity of Ammonia-Oxidizing Archaea in the Surface Sediments of the Northern Continental Slope of the South China Sea

LIU Guohui1,3,WU Houbo2   

  1. (1.Key Laboratory of Marginal Sea Geology,South China Sea Institute of Oceanology,the Chinese Academy of Sciences,Guangzhou 510301,China; 2.Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,the Chinese Academy of Sciences, Guangzhou 510301,China;3.University of Chinese Academy of Sciences,Beijing 100049,China)
  • Online:2016-01-05 Published:2016-01-05

Abstract: Surface sediment samples were taken from three sites of different water depths at the northern continental slope of the South China Sea to investigate the community structure of ammonia-oxidizing archaea (AOA). Polymerase chain reaction (PCR) was employed to amplify the archaeal ammonia monooxygenase α subunit (amoA) gene of AOA. Amplicons of the amoA gene sequences were used to understand the diversity and phylogenetic relationship of AOA. The main results were as follows: 1) All of the archaeal amoA gene sequences from the three locations were affiliated to Group I.1a Thaumarchaeota; 2) all of the archaeal amoA gene sequences from the three locations were phylogenetically closely related; and 3) the 16S rRNA gene and amoA gene phylogenetic trees were of congruent topology. Thaumarchaeota accounted for 40% of the total archaea, indicating that AOA might play an important role in the nitrogen biogeochemical cycling in the surface sediments of the northern continental slope of the South China Sea.

Key words: Ammonia-Oxidizing Archaea, amoA gene, surface sediment, northern South China Sea