In the Karst peak forest-plain area of Jiulong Town in Yingde, Guangdong, six land use types, namely slope shrubs, water depressions，sugar-orange forests, bamboo groves, vegetable patches and rice fields, were selected to be studied, their soil at different depth were sampled, and then the total calcium content, the exchangeable calcium content, and the water soluble calcium content were measured by atomic absorption spectrophotometer. The results showed that: 1) The thickness of the soils in vegetable patches, rice fields, sugar-orange forests and bamboo groves were significantly greater than that of natural slope shrubs and water depressions; 2) The total calcium content of soil in Karst water environment (water depressions, rice fields) was over 59 g/kg on average, which was the highest among the six land use types, the second was that of the soil on slope, which was 20.83 g/kg. In the cultivated environment of sugar-orange forests and vegetable patches, the total calcium content reduced to 3.51 and 2.76 g/kg, respectively. By contrast, in the adjacent non-karst regions, total calcium content of soil was only 1.43 g/kg. 3) Because cultivating and fertilizing would increase the exchangeable calcium content and the water soluble calcium of soil, the proportion of exchangeable calcium and water soluble calcium in the total calcium content were, respectively, 38.04%, 23.08% for vegetable patches, 1.41%, 0.88% for sugar-orange forests, 7.86%, 6.87% for water depressions, and 0.25%, 0.46% for slope shrubs. Since the total calcium was reducing in vegetable patches and sugar-orange forests, the increase of exchangeable calcium and water soluble calcium would aggravate the calcium loss. 4) The variations of total calcium, exchangeable calcium and soluble calcium contents with soil depth showed a decreasing trend under different land use types, however, the variations in water depression and rice fields were much greater than those in vegetable patches, sugar-orange forests and bamboo groves. 5) The thickness of soil, bare ratio of rock and slope were significantly negatively correlated with each other, while a marked positive correlation existed among total calcium, exchangeable calcium and water soluble calcium in soil, and also a significant negative correlation existed between bare ratio of rock and exchangeable calcium. In conclusion, water, soil thickness, bare ratio of rock and slope jointly determined the distribution and migration of soil calcium.